| Autor: |
Trisha I. Valerio, Feng Yan, Jayla A. Wilson, Maegan M. Watson, Chen Wang, Michael T. OConnell, Gokhan Gunay, Handan Acar, Qinggong Tang |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Biomedical Applications of Light Scattering XI. |
| DOI: |
10.1117/12.2578750 |
| Popis: |
Three-dimensional (3D) cell culture models are developed as a promising platform to screen anticancer therapeutics and treatments. However, current imaging techniques cannot provide 3D structures of tumor spheroids in situ. In this study, we employed label-free and noninvasive optical coherence tomography (OCT) for imaging and quantifying the 3D structures of tumor spheroids. We imaged ovarian cancer spheroids with OVCAR-8 cell line over a period of 10 days with 5,000 and 50,000 initial cell numbers. We successfully reconstructed the 3D necrotic regions via label-free intrinsic scattering attenuation contrast and evaluated the effect of Cisplatin treatment on tumor spheroids. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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