Determination of Compartmented Metabolite Pools by a Combination of Rapid Fractionation of Oat Mesophyll Protoplasts and Enzymic Cycling
| Autor: | Rüdiger Hampp, Marion Goller, Helene Füllgraf |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Chromatography
food.ingredient biology Physiology Metabolite fungi food and beverages Plant Science Fractionation biochemical phenomena metabolism and nutrition Mitochondrion Protoplast Chloroplast chemistry.chemical_compound Avena food Biochemistry chemistry In vivo Genetics biology.protein Citrate synthase |
| Zdroj: | Plant Physiology. 75:1017-1021 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.75.4.1017 |
| Popis: | In vivo pool sizes of a range of metabolites have been determined in subcellular fractions of darkened and illuminated mesophyll protoplasts of Avena sativa L. These estimations were made by combining a method of rapid protoplast fractionation with enzymic cycling techniques. Results are given for reduced and oxidized pyridine nucleotides, triose phosphates, 3-phosphoglycerate, inorganic phosphate, aspartate, malate, oxaloacetate, glutamate, 2-oxoglutarate, and citrate, from chloroplasts, mitochondria, and a fraction representing the remainder of the protoplast. The results indicate distinct differences of compartmented levels of certain metabolites between darkened and illuminated protoplasts. |
| Databáze: | OpenAIRE |
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