CHLOROPLAST PIGMENTS AND PHOTOSYNTHESIS IN DEUTERATED GREEN ALGAE*

Autor: Mary R. Thomas, H. H. Strain, Henry L. Crespi, Joseph J. Katz, Martin I. Blake
Rok vydání: 2006
Předmět:
Zdroj: Annals of the New York Academy of Sciences. 84:617-633
ISSN: 0077-8923
DOI: 10.1111/j.1749-6632.1960.tb39095.x
Popis: The isolation and characterization of deuteriochlorophylls a and b from the chloroplasts of fully deuterated green algae, together with some preliminary observations on photosynthesis in deuterated algae, are described. An improved procedure is given for the isolation and purification of the chlorophylls. The algae used in the study were cultured for more than a year in a nutrient solution prepared from 99.8% D/sub 2/0. The infrared studies reported provide independent evidence that the chlorophyll made by the algae is essentially free of hydrogen. The ratio of deuterio-chlorophyll a to b in crude extracts of the deuterated algae was determined to be in the range 1.5: 1 to 2: 1. The uptake of NaHC/sup 14/ O/sub 3/ by ordinary and deuterated C. vulgaris in H/sub 2/0 and D/sub 2/0 were measured, and the results are tabulated. (P.C.H.)
Databáze: OpenAIRE