Detection of the biotin–streptavidin interaction by exploiting surface stress changes on ultrathin Si membranes
| Autor: | Stavros Chatzandroulis, Dimitris Kafetzopoulos, Christos Boutopoulos, D. Goustouridis, Ioanna Zergioti, M. Ioannou, P. Andreakou, Pascal Normand, Dimitris Tsoukalas, V. Tsouti |
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| Rok vydání: | 2009 |
| Předmět: |
Streptavidin
Chemistry Surface stress technology industry and agriculture Nanotechnology Condensed Matter Physics Capacitance Atomic and Molecular Physics and Optics Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Membrane Biotin Fluorescence microscope Electrical and Electronic Engineering |
| Zdroj: | Microelectronic Engineering. 86:1495-1498 |
| ISSN: | 0167-9317 |
| DOI: | 10.1016/j.mee.2008.11.058 |
| Popis: | The detection of the biotin-streptavidin interaction is presented based on surface stress changes of functionalized Si membranes. During the biomolecular interaction, the surface stress of the functionalized membranes alters resulting in their deflection and finally in capacitance variations. The biological interaction on the sensors surface was verified after each experiment using fluorescent microscopy. Successful detection of 21nM of streptavidin using 3.55mM biotin probes is presented. |
| Databáze: | OpenAIRE |
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