| Popis: |
1. 1. Albumins from adult swine serum, foetal serum, colostrum and urine from neonatal pigs were isolated by the use of immunosorbent techniques. 2. 2. The purity of the isolated albumins was controlled by polyacrylamide gel-electrophoresis, electroimmunoassay and by immunization of rabbits with isolated albumin. 3. 3. Gel-filtration showed that the mol. wts of adult serum albumin, foetal serum albumin and colostrum albumin were 67,500, 66,500 and 65,000, respectively. Urine albumin was shown to be composed of 2 populations with mol. wts of 65,500 and 44,500. Determination of the sedimentation coefficients confirmed these results. 4. 4. Albumin from neonatal pig urine has an amino acid composition intermediary to that of colostrum and foetal serum, while a similar composition was found for colostrum and adult serum albumin. 5. 5. Isoelectric focusing showed a similar pI-pattern for adult and foetal serum albumin on the one hand and urine and colostrum albumin on the other hand while neonatal serum albumin showed an intermediate pattern. 6. 6. The data obtained confirmed previous findings that albumin in colostrum originates from sow's serum. After onset of colostrum ingestion colostrum albumin is transported via the intestinal absorptive cells to the blood of the neonatal pig and is then excreted into the urine during the period of transient proteinuria. |
