Affinity sorbent based on tryptophyl-threonyl-tyrosine for binding of the immunoglobulins G: Sorption characteristics and aspects of practical application

Autor:	M. I. Afanas’eva, P. A. Levashov, A. A. Az’muko, E. D. Ovchinnikova, I. Yu. Adamova, Zh. D. Bespalova, S. N. Pokrovskii, D. A. Frid, O. I. Afanas’eva
Rok vydání:	2012
Předmět:	Sorbent Chromatography biology Chemistry Organic Chemistry Extraction (chemistry) Sorption Tripeptide Biochemistry Immunoglobulin G Matrix (chemical analysis) Affinity chromatography biology.protein Bioorganic chemistry
Zdroj:	Russian Journal of Bioorganic Chemistry. 38:46-50
ISSN:	1608-330X 1068-1620
Popis:	Chromatographic material based on tryptophil-threonyl-tyrosine and efficiently binding human, sheep, goat, and bovine immunoglobulin G was obtained. High selectivity of the sorbent for extraction of immunoglobulins from blood plasma has been demonstrated. Effective sorption capacity is 15–25 mg of immunoglobulin G per 1 ml of the matrix. Optimization of the method of triptophyl-threonyl-tyrosine covalent binding to the polysaccharide matrix allowed the achievement of high sorbent stability in conditions of use and storage. This sorbent may be used in medicine and biotechnology.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9bf25eb5598a7bc24efc322d4d408309 https://doi.org/10.1134/s1068162012010141 Zobrazit plný text záznamu Full text from SpringerLink