Design of a two-photon fluorescent probe for ratiometric imaging of endogenous hypochlorite in mitochondria
| Autor: | Xiangming Meng, Min Fang, Linke Li, Xinru Wang, Tingting Xu, Zhijian Yang, Yan Feng, Xin Wang, Chuan Sun, Man Chen, Zhengsong Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
Analyte
Process Chemistry and Technology General Chemical Engineering Hypochlorite Endogeny 02 engineering and technology Mitochondrion 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence Dual response 0104 chemical sciences chemistry.chemical_compound chemistry Two-photon excitation microscopy Mitochondrial targeting Biophysics 0210 nano-technology |
| Zdroj: | Dyes and Pigments. 181:108548 |
| ISSN: | 0143-7208 |
| DOI: | 10.1016/j.dyepig.2020.108548 |
| Popis: | Hypochlorite (ClO−) is associated with complex physiological activities in the living system. Therefore, it is necessary to develop a tool for visualizing ClO− at the cellular and subcellular levels. We herein presented a two-photon fluorescent probe MCL with dual response sites and ratiometric response to ClO−. The response sites of two oxathiolane rings endowed probe MCL with superior selectivity for ClO− over other analytes. The fast response and high sensitivity of MCL to ClO− were also well demonstrated. In addition, probe MCL was confirmed to possess accurate mitochondrial targeting ability through a co-localization study. The good two-photon sensing performance of MCL for exogenous and LPS-induced endogenous ClO− in living cells was validated by dual-color imaging. Moreover, we employed the probe to successfully monitor endogenous ClO− in H2S-stimulated cells by dual-channel mode for the first time. |
| Databáze: | OpenAIRE |
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