Electrochemical behavior of redox proteins entrapped in nafion films

Autor:	Pierre Bianco, Jean Haladjian
Rok vydání:	1994
Předmět:	chemistry.chemical_compound Chemistry Nafion Electrode Inorganic chemistry Electrochemistry Molecule Pyrolytic carbon Voltammetry Redox Ferredoxin Analytical Chemistry
Zdroj:	Electroanalysis. 6:415-422
ISSN:	1521-4109 1040-0397
Popis:	Several redox proteins are studied at Nafion-coated pyrolytic graphite (PG) electrodes. The electrochemical behavior of polyheme c-type cytochromes and spinach ferredoxin is described using cyclic and square-wave voltammetry (SWV). No signals are detected when Nafion-coated electrodes are exposed to the protein solutions. In contrast, electrochemical responses are observed when droplets of protein solutions, deposited on the PG surface are dried then entrapped in Nafion layer. These Nafion-modified electrodes permit one to detect very small amounts (1 μg) of cytochrome c3. The stability of entrapped molecules upon repetitive cycling has been investigated. Such modified electrodes offer new perspectives for studying proteins confined at electrode surfaces.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9be4d254e8a83b1a25355091ed9cd1ae https://doi.org/10.1002/elan.1140060510 Zobrazit plný text záznamu Plný text