Automated analysis for vitamin E in butter by coupling sample treatment – continuous membrane extraction – liquid chromatography with electrochemical detection
| Autor: | A. Sanchez Perez, M Bustamante Rangel, M. M. Delgado Zamarreño, J. Hernández Méndez |
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| Rok vydání: | 1999 |
| Předmět: |
Chromatography
Chemistry Coefficient of variation Vitamin E medicine.medical_treatment Extraction (chemistry) Analytical chemistry Biochemistry Analytical Chemistry Membrane technology Membrane medicine Environmental Chemistry Dissolution Quantitative analysis (chemistry) Alkaline hydrolysis Spectroscopy |
| Zdroj: | Analytica Chimica Acta. 386:99-106 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/s0003-2670(99)00014-8 |
| Popis: | An automated procedure for the determination of vitamin E in samples of butter with minimum sample manipulation has been developed. Following dissolution of the samples in a micellar medium (methanol–Triton X-100–water), on-line sample treatment involving alkaline hydrolysis is coupled with a separation and enrichment system in a cell containing a silicone membrane; the analyte-rich phase is finally connected to a liquid chromatographic analytical system – using a C18 column – with electrochemical detection controlling the whole system automatically. The proposed procedure has been successfully applied to the determination of vitamin E in samples of butter, using 2,2,5,7,8-pentamethyl-6-chromanol as internal standard. The coefficient of variation obtained was 6.7% for a inter-day assay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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