Isolation, Expression Pattern, Polymorphism and Association Analysis of Porcine TIAF1 Gene

Autor:	Y. Wang, M. G. Lei, B. Zuo, Z. H. Q. Ren, Chang-Yan Deng, Y. Z. Xiong
Rok vydání:	2009
Předmět:	TIAF1 Gene education.field_of_study Mutation Marbled meat Population Biology medicine.disease_cause Molecular biology Exon Gene expression medicine Animal Science and Zoology Intramuscular fat Allele education Food Science
Zdroj:	Asian-Australasian Journal of Animal Sciences. 22:313-318
ISSN:	1976-5517 1011-2367
DOI:	10.5713/ajas.2009.80400
Popis:	TIAF1 is a TGF-β1-induced anti-apoptotic factor that plays a critical role in blocking TNF (tumor necrosis factor) cytotoxicity in mouse fibroblasts and participates in TGF-β-mediated growth regulation. In this study, we obtained the full-length cDNA sequence of the porcine TIAF1 gene. Real-time PCR further revealed that the TIAF1 gene was expressed at the highest level in liver and kidney with prominent expressions detected in uterus, and lower levels detected in heart, spleen, lung, stomach, small intestine, skeletal muscle and fat of Large White pigs. Sequence analysis indicated that a 6 base-pair deletion mutation existed in the exon of the TIAF1 gene between Meishan and Large White pigs. This mutation induced deletion of Gln and Val amino acids. PCR-RFLP was used to detect the polymorphism in 394 pigs of a "Large WhitexMeishan" F 2 resource population and four purebred pig populations. The frequencies of the A allele (with a 6 bp deletion) were dominant in Chinese Meishan and Bamei pigs, and the frequencies of the B allele (no 6 bp deletion) were dominant in Large White and Landrace pigs. Association analyses revealed that the deletion mutation had highly significant associations (p
Databáze:	OpenAIRE
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