| Autor: |
Taewoong Son, Youn-Joo Cho, Hyunseung Lee, Mi Young Cho, Byeongwoo Goh, Hyun Min Kim, Hoa Thi Ngoc Phan, Sun-Hee Cho, Young-Jun Park, Hye Sun Park, Kwan Soo Hong |
| Rok vydání: |
2022 |
| DOI: |
10.21203/rs.3.rs-1230726/v1 |
| Popis: |
Background: There has been growing concern regarding the impact of air pollution, especially fine dust, on human health. However, it is difficult to estimate the toxicity of fine dust on the human body because of its diverse effects depending on the composition and environmental factors.Results: In this study, we focused on the difference in the biodistribution of fine dust according to the size distribution of particulate matter after inhalation into the body to predict its impact on human health. We synthesized Cy7-doped silica particulate matters (CSPMs) having different particle sizes and employed them as model fine dust, and studied their whole-body in vivo biodistribution in BALB/c nude mice. Image-tracking and quantitative analysis were performed on the ex vivo organs and tissues. Additionally, flow cytometric analysis of single cells isolated from the lungs was performed. Smaller particles with a diameter of less than 100 nm (CSPM0.1) were observed to be removed relatively rapidly from the lungs upon initial inhalation. However, they were confirmed to accumulate continuously over 4 weeks of observation. In particular, smaller particles were found to spread rapidly to other organs during the early stages of inhalation.Conclusions: It is expected that the effect of fine dust on human health can be predicted through the differences in in vivo behavior that arise depending on the particle size. This study might provide with insights on association between CSPM0.1 accumulation in several organs including the lungs and adverse effect to underlying diseases in the organs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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