Comparative effects of two different doses of low-level laser therapy on wound healing third-degree burns in rats
| Autor: | Maria Carolina Derencio Oliveira, Carla Roberta Tim, Fernando M. Araújo-Moreira, Paulo Sérgio Bossini, Patricia Brassolatti, Lucimar Retto da Silva de Avó, Nivaldo Antonio Parizotto, Hueliton Wilian Kido, Luciana Almeida-Lopes |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Histology Third-Degree Burn Indirect contact Angiogenesis business.industry medicine.medical_treatment Urology 030206 dentistry Microcirculation Surgery 030207 dermatology & venereal diseases 03 medical and health sciences Medical Laboratory Technology 0302 clinical medicine Laser therapy medicine Anatomy Wound healing business Instrumentation Immunostaining Low level laser therapy |
| Zdroj: | Microscopy Research and Technique. 79:313-320 |
| ISSN: | 1059-910X |
| Popis: | Burns are injuries caused by direct or indirect contact to chemical, physical, or biological agents. Low-level laser therapy (LLLT) is a promising treatment since it is low-cost, non-invasive, and induces cell proliferation. This study aimed to investigate the effects of LLLT (660 nm) at two different fluences (12.5 J/cm(2) and 25 J/cm(2) ) per point of application on third-degree burns in rats. Thirty rats (Wistar) divided into GC, GL12.5, and GL25 were used in the study, and submitted to burn injury through a soldering iron at 150°C, pressed on their back for 10 s. LLLT was applied immediately, and 2, 4, 6, and 8 days after wound induction. Histological analysis revealed a decreased inflammatory infiltrate in the group treated with 25 J/cm(2) , and intense inflammatory infiltrate in the control group and in the group treated with 12.5 J/cm(2) . The immunostaining of COX-2 was more intense in the control groups and in the group treated with 12.5 J/cm(2) than in the group treated with 25 J/cm(2) . Conversely, VEGF immunomarking was more expressive in the group treated with 25 J/cm(2) than it was in the other two groups. Therefore, our findings suggest that the use of 25 J/cm(2) and 1 J of energy was more effective in stimulating the cellular processes involved in tissue repair on third-degree burns in rats by reducing the inflammatory phase, and stimulating angiogenesis, thus restoring the local microcirculation which is essential for cell migration. |
| Databáze: | OpenAIRE |
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