Reactive Oxygen Species in The Photosensitization of Retinal Pigment Epithelial Cells by Rose Bengal
| Autor: | J. Diane Wiltshire, I. Aravind Menon, Suruj D. Persad, Arpita Das, Prasanta K. Basu |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species biology Singlet oxygen Superoxide Health Toxicology and Mutagenesis chemical and pharmacologic phenomena Glutathione Toxicology Photochemistry Molecular biology Superoxide dismutase Ophthalmology chemistry.chemical_compound chemistry Rose bengal biology.protein Sodium azide Photosensitizer |
| Zdroj: | Journal of Toxicology: Cutaneous and Ocular Toxicology. 11:269-283 |
| ISSN: | 0731-3829 |
| Popis: | Rose bengal (RB) is a fluorescein derivative used as a vital stain in certain ophthalmic diagnostic procedures. RB is a potent photo-sensitizer and has been used as a model for photosensitized reactions involving singlet oxygen. Ultraviolet (UV)-visible (UV-VIS) irradiation of RB with nitroblue tetrazolium (NBT) in the absence of glutathione (GSH) induced the reduction of NBT. However since superoxide dismutase (SOD) did not inhibit this NBT reduction it seems to be not mediated by superoxide, but due to the direct reduction of NBT by the excited state of RB. UV-VIS irradiation of RB, NBT, and 1-5 mM GSH reduced larger amounts of NBT. Moreover, SOD partially inhibited the NBT reduction under these conditions, indicating the formation of superoxide. The optimal pH for the formation of superoxide was 7-9. The superoxide formation during UV-VIS irradiation of RB and GSH was not inhibited by the singlet oxygen scavengers β-carotene, sodium azide, or 1,4-diaza-bicyclo[2.2.2]octane (DABCO), indicating t... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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