Hydrangea mosaic virus, a new ilarvirus from Hydrangea macrophylla (Saxifragaceae)

Autor: B. J. Thomas, Anna Tuszynski, R. J. Barton
Rok vydání: 1983
Předmět:
Zdroj: Annals of Applied Biology. 103:261-270
ISSN: 1744-7348
0003-4746
DOI: 10.1111/j.1744-7348.1983.tb02763.x
Popis: SUMMARY A previously unrecognised virus isolated from Hydrangea macrophylla with chlorotic mosaic leaf symptoms in West Sussex was named hydrangea mosaic virus (HydMV). HydMV was mechanically transmitted without difficulty to four of 16 species from three of five families, and was seed transmitted in Chenopodium quinoa, but was not aphid transmitted. Although relatively unstable in vitro, HydMV was purified by clarifying leaf extracts by emulsification with chloroform and acidification with citric acid, followed by differential centrifugation and sucrose density gradient centrifugation. Purified virus incompletely separated on sucrose density gradients into three components (T. M and B) with sedimentation coefficients (so20w) of 86, 97, and 105 S respectively, but all particles had buoyant densities in caesium chloride of 1.37 g/cm3. Virus contained a single polypeptide species (mol. wt 26.4 times 103), appeared quasiisometric to ovoid or elliptical, and measured c. 28 times 30 (T), 30 times 30 (M) or 30 times 32–38 nm (B). Single-stranded RNA species or mol. wt 1–25, 1–08, 0–83, 0–36 and 0–27 (RNA-1 to 5 respectively) were obtained from virus preparations but mixtures containing RNA-1 to 3 plus either RNA-4 + 5 or the coat protein, were infective. These properties suggest that HydMV has affinities with ilarviruses, but it showed no serological relationship to any of six ilarviruses or 42 other viruses.
Databáze: OpenAIRE
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