Isolation and Characterization of Mercury ResistantBacillussp. from Soils with an Extensive History as Substrates for Mercury Extraction in Mexico
Autor: | Julio Alfonso Cruz Medina, Jessica Enriquez Farias, George H. Jones, Sara Solis Valdes, Rocío García Martínez, Juan Campos-Guillén, Andrés Cruz Hernández, Gilberto Hernández Silva |
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Rok vydání: | 2013 |
Předmět: |
biology
Inoculation fungi food and beverages chemistry.chemical_element 16S ribosomal RNA biology.organism_classification Rhizobacteria Microbiology Mercury (element) Phytoremediation Horticulture Bioremediation chemistry Shoot Botany Earth and Planetary Sciences (miscellaneous) Environmental Chemistry Bacteria General Environmental Science |
Zdroj: | Geomicrobiology Journal. 30:454-461 |
ISSN: | 1521-0529 0149-0451 |
Popis: | Metal phytoextraction assisted by bacteria plays an important role in bioremediation systems. In this work, mercury-resistant bacterial strains were isolated from soils with high levels of mercury (San Joaquin, Queretaro State, Mexico) and identified as Bacillus sp. based on the 16S rDNA gene sequence analysis. The bacterial strains were found to exhibit different multiple mercury-resistance and carbon source utilization characteristics. The mercury reduction ability was tested through a volatilization assay. The bacterial isolates were also evaluated for their ability to promote growth and mercury uptake in tomato plants. In a roll towel assay, the maximum vigor index of tomato plants was obtained with the inoculation of Bacillus sp. A2, A12, B11, B15 and C1, while in a pot assay, the maximum vigor index was obtained with the inoculation of Bacillus sp. A6, A7 and B20, compared with un-inoculated controls in the presence of HgCl2. Maximum Hg accumulation in the roots and shoots of tomato plants was obtai... |
Databáze: | OpenAIRE |
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