| Autor: |
Kamila Delaney, Caroline Gabus, Françoise Schwager, Joanna M. Wenda, Florian A. Steiner, Monica Gotta, Reinier F Prosée |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
Centromere protein A (CENP-A) is a histone H3 variant that defines centromeric chromatin and is essential for centromere function. In most eukaryotes CENP-A-containing chromatin is epigenetically maintained, and centromere identity is inherited from one cell cycle to the next. In the germ line of the holocentric nematodeCaenorhabditis elegans, this inheritance cycle is disrupted. CENP-A is removed at the mitosis-to-meiosis transition and is establishedde novoon chromatin during diplotene of meiosis I. Here we show that the N-terminal tail of CENP-A is required for thede novoestablishment of centromeres, but dispensable for centromere maintenance during embryogenesis. Worms homozygous for a CENP-A tail deletion maintain a functional centromere during development, but give rise to inviable offspring because they fail to re-establish centromeres in the maternal germ line. We identify the N-terminal tail of CENP-A as a critical domain for the interaction with the conserved kinetochore protein KNL-2, and argue that this interaction plays an important role in setting centromere identity in the germ line. We conclude that centromere establishment and maintenance are functionally distinct inC. elegans. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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