Improving scFv antibody expression levels in the plant cytosol1
| Autor: | Jaap Bakker, Dirk Bosch, Jan M. de Boer, Annemiek Wilmink, Marie-Noëlle Rosso, Willem J. Stiekema, Jan Roosien, A. Schouten, Fred J. Gommers, Arjen Schots |
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| Rok vydání: | 1997 |
| Předmět: |
0106 biological sciences
Regulation of gene expression 0303 health sciences Chemistry Endoplasmic reticulum Transgene KDEL fungi Biophysics food and beverages ER retention Cell Biology 01 natural sciences Biochemistry 03 medical and health sciences Cytosol N-linked glycosylation Structural Biology Genetics KDEL Motif Molecular Biology 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | FEBS Letters. 415:235-241 |
| ISSN: | 0014-5793 |
| Popis: | Expression of single-chain antibody fragments (scFvs) in the plant cytosol is often cumbersome. It was unexpectedly shown that addition at the C-terminus of the ER retention signal KDEL resulted in significantly improved expression levels. In this report the cytosolic location of the scFv-CK was confirmed, excluding possible mistranslocation to other subcellular compartments. It was shown that expression of several other scFvs was also improved in tobacco protoplasts. In addition expression was improved in transgenic potato. Changing from KDEL to KDEI did not affect the enhanced protein expression level. Addition of the KDEL motif is a simple and straightforward tool to stabilize in planta cytosolic expression of many scFvs. |
| Databáze: | OpenAIRE |
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