Effect of Topographical Steps on the Surface Motility of the Bacterium Pseudomonas aeruginosa
| Autor: | Daniel L. Barton, Eric R. Weeks, Jure Dobnikar, William A. Ducker, Yow-Ren Chang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cell diameter
Surface (mathematics) biology Chemistry Pseudomonas aeruginosa 0206 medical engineering Biomedical Engineering Step height Biofilm Motility 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease_cause biology.organism_classification 020601 biomedical engineering Biomaterials Fluorescence microscope Biophysics medicine 0210 nano-technology Bacteria |
| Zdroj: | ACS Biomaterials Science & Engineering. 5:6436-6445 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.9b00729 |
| Popis: | Bacteria traverse surfaces as part of colonizing solids, and it is of interest to hinder this motion to potentially thwart infections in humans. Here, we demonstrate that topographical steps hinder the ability of Pseudomonas aeruginosa PAO1 (P. aeruginosa) to traverse a solid-liquid interface. Using time-lapse fluorescence microscopy and image analysis, we analyzed the motion of P. aeruginosa that were challenged with steps ranging in height from 0.4 to 9.0 μm. Bacterial trajectories are sensitive to the height of the step, the curvature of the step face, and the direction of their motion relative to gravity. When the step height is ≥0.9 μm, which is similar to the cell diameter, there is a reduced probability of the cell crossing the step. For those bacteria that do cross a step, there is a time penalty for crossing steps of height 2-3 μm; this height is similar to the length of the bacterium. For higher steps, the bacteria reorient their cell body while traversing the step riser. Our findings elucidate how topography influences the motion of bacteria and informs strategies for hindering bacterial motion at surfaces. |
| Databáze: | OpenAIRE |
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