| Autor: |
R Mukai, R K Hamatake, M Hayashi, A Aoyama |
| Rok vydání: |
1983 |
| Předmět: |
|
| Zdroj: |
Journal of Biological Chemistry. 258:5798-5803 |
| ISSN: |
0021-9258 |
| DOI: |
10.1016/s0021-9258(20)81964-4 |
| Popis: |
The product of gene C of bacteriophage phi X174 is required for the replication of phiX174 single-stranded DNA in Escherichia coli cells infected with phi X174. The protein has been purified to homogeneity using an in vitro complementation system. The protein exhibits a molecular weight of 5800 under denaturing conditions. The NH2-terminal amino acid sequence of the protein is (NH2)-Met-Arg-Lys, which is consistent with the sequence predicted from the nucleotide sequence of gene C. The protein has an affinity for single-stranded DNA but less for double-stranded DNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
