Microglial activation and neurobiological alterations in experimental autoimmune prostatitis-induced depressive-like behavior in mice
| Autor: | Zi-Qiang Yu, Yi Liu, Chaozhao Liang, Jin Zhang, Li Zhang, Chang-Sheng Zhan, Yong Zhang, Kai Zhong, Jing Chen, Hexi Du, Yang Hongyi, Xianguo Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Microglia Glial fibrillary acidic protein biology business.industry Glutamate receptor Tail suspension test 030227 psychiatry Proinflammatory cytokine Baicalein 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure Endocrinology chemistry Internal medicine medicine biology.protein Neurotransmitter metabolism business 030217 neurology & neurosurgery Neuroinflammation |
| Zdroj: | Neuropsychiatric Disease and Treatment. 15:2231-2245 |
| ISSN: | 1178-2021 |
| DOI: | 10.2147/ndt.s211288 |
| Popis: | Background Patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) frequently show depressive symptoms clinically and increasing evidence indicates a correlation between CP/CPPS and depression. However, the underlying mechanisms of CP/CPPS-related depression remain poorly understood. Here, we sought to determine the role of hippocampal microglial activation and neurobiological changes in a mouse model of experimental autoimmune prostatitis (EAP)-induced depression and the treatment efficacy of Chinese herb extract baicalein. Methods EAP was induced through intradermal injection of prostate antigen and adjuvant twice. Then, mice were assessed for affective behaviors in the open field test, elevated plus maze, forced swim test, and tail suspension test. The morphology and function of microglia and astrocytes were detected by immunofluorescence, Western blotting, and transmission electron microscopy. Proinflammatory mediators along with serotonin transporter (SLC6A4/SERT) and indoleamine 2,3-dioxygenase (IDO) were quantified with reverse transcription-polymerase chain reaction (RT‑PCR), and serum serotonin concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Proton magnetic resonance spectroscopy (1H-MRS) was performed to measure hippocampal glutamate levels. In addition, baicalein was used in a subset of EAP mice to test its anti-depressant action. Results EAP was successfully established and induced depressive- and anxiety-like behavior in mice. Increasing levels of co-expressed ionized calcium-binding adapter molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) and ultrastructural observations suggested microglial activation and reactive astrocytosis in the hippocampus. These activated microglia resulted in increased expressions of multiple proinflammatory cytokines. Simultaneously, EAP mice showed higher gene expressions of SLC6A4 and IDO and lower concentrations of serotonin. 1H-MRS indicated a decrease in the glutamate + glutamine (Glx)/total creatine (tCr) ratio in EAP mice. Furthermore, baicalein treatment alleviated the depressive-like behavior and neuroinflammation by suppressing the nuclear factor-kappa B (NF-κB) pathway. Conclusion Our data indicate that EAP-induced depressive-like behavior is linked to microglia activation and subsequent neurotransmitter metabolism. Moreover, baicalein attenuates behavioral changes by inhibiting neuroinflammation via downregulation of the NF-κB pathway. |
| Databáze: | OpenAIRE |
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