Analysis of nitrofuran metabolic residues in salt by liquid chromatography–tandem mass spectrometry
| Autor: | L.C Pampana, Alberto dos Santos Pereira, G. De Nucci, J. L. Donato |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Semicarbazide
Nitrofurazone Chromatography medicine.drug_class Metabolite Mass spectrometry Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Liquid chromatography–mass spectrometry medicine Environmental Chemistry Derivatization Nitrofuran Spectroscopy |
| Zdroj: | Analytica Chimica Acta. 514:9-13 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2004.03.033 |
| Popis: | Recent reports from the Brazilian Agricultural Ministry program of residue control describe some cases of the presence of semicarbazide, the target metabolite of nitrofurazone {2-[(5-nitro-2-furanyl)methylene]-hydrazinecarboxamide}, in samples of salted chicken meat. Since that contamination can be originated from other reasons in addition to nitrofurazone abuse, our laboratory has developed a sensitive and simple method for detection of four nitrofuran metabolites in salt samples. This method include a previous hexane extraction of the metabolites followed by a derivatization reaction with 2-nitrobenzaldehyde. The nitrophenyl derivatives were analyzed by liquid chromatography–tandem mass spectrometry without any additional clean-up procedures. The limit of quantification for routine analysis was 0.5 μg kg −1 for each metabolite. |
| Databáze: | OpenAIRE |
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