| Autor: |
Akram Eidi, Fattah Sotoodehnejadnematalahi, Mojtaba Khaksarian, Mahdie Ahmadi, Hassan Ahmadvand |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.21203/rs.3.rs-430600/v1 |
| Popis: |
Carvacrol is a phenolic monoterpene constituent of essential oils produced by plants such as Nigella sativa. This study examined the therapeutic effects of carvacrol in a model of experimental autoimmune encephalomyelitis (EAE). EAE induction was performed on female Lewis rats and, after appearance of the first clinical signs, a second EAE group received carvacrol intraperitoneally each day for 17 days. Clinical symptoms and body weight were assessed daily. All animals sacrificed and histological and gene expression analysis of the spinal cord were performed. The carvacrol treated group scored lower for disease after therapeutic administration than the control group. Gene expression analysis on the carvacrol treated group showed a decrease in TNF-α, NF-ҚB and IL-17 (pro-inflammatory) gene expression and an increase in MBP and OLIG2 remyelination markers. In the carvacrol treated group, H&E stain of spinal cord sections revealed a remarkable decrease in the amount of inflammatory cell infiltration. Immunostaining of lumbar spinal cord sections demonstrated a remarkable increase in MBP and a decrease in IL-17 secretion in the carvacrol treated group. These results demonstrate that carvacrol had a beneficial therapeutic effect and anti-inflammatory properties that significantly reduced the clinical scores and promoted myelin repair. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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