Differential synthesis of RNA in self- and cross-pollinated styles of Petunia hybrida L

Autor:	J. A. W. M. van der Donk
Rok vydání:	1974
Předmět:	chemistry.chemical_compound chemistry Polyacrylamide Gene expression Genetics RNA Biology Molecular Biology Molecular biology Polyacrylamide gel electrophoresis Petunia hybrida
Zdroj:	Molecular and General Genetics MGG. 131:1-8
ISSN:	1432-1874 0026-8925
DOI:	10.1007/bf00269381
Popis:	Styles of Petunia hybrida were pollinated (selfed or crossed) and pollentubes were allowed to grow in the presence of 3H-orotic acid. After 24 h RNA was extracted using the cold phenol method and separated on a tandem polyacrylamide gel. Results show that: 1. the synthesis of RNA is more enhanced after cross- than after selfpollination and 2. a restricted number of RNA peaks is responsible for this difference; this RNA has a molecular weight different from r-RNA and t-RNA and could be messenger-like of nature. Results indicate a differential gene expression correlated with rejection or acceptance of the pollentubes by the style.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9b08696d86aeef07e0a274736e5a3e0a https://doi.org/10.1007/bf00269381 Zobrazit plný text záznamu Full text from SpringerLink