Antiviral Activity Against Chikungunya Virus of Diterpenes from the Seaweed Dictyota menstrualis
| Autor: | Claudio Cesar Cirne-Santos, Max Willian Lisboa Gomes, Diana Negrão Cavalcanti, Roberto Campos Villaça, Rafaela dos Santos Pereira Gomes, Valéria Laneuville Teixeira, Johana Marcela Concha Obando, Izabel Christina Nunes de Palmer Paixão, Caroline de Souza Barros, Priscilla O. Esteves, Carlos José Brito Ramos |
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| Rok vydání: | 2020 |
| Předmět: |
Infectivity
Drug 010405 organic chemistry viruses Ribavirin Dictyota menstrualis media_common.quotation_subject medicine.disease_cause 01 natural sciences Virology Virus 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry.chemical_compound chemistry Viral replication medicine Chikungunya General Pharmacology Toxicology and Pharmaceutics EC50 media_common |
| Zdroj: | Revista Brasileira de Farmacognosia. 30:709-714 |
| ISSN: | 1981-528X |
| DOI: | 10.1007/s43450-020-00083-9 |
| Popis: | Chikungunya virus infection has become one of the most challenging re-emergent viral infections. Given that there is as yet no vaccine or effective drugs against chikungunya virus, our group evaluated products from Dictyota menstrualis (Hoyt) Schnetter, Horning, & Weber-Peukert 1987, Dictyoteae, for their antiviral potential. We evaluated whether crude extracts, acetylated crude extracts, and their fractions could inhibit chikungunya virus replication. The F-5 and FAc-1 fractions, rich in cyclic diterpenes with aldehyde groupings, inhibited replication by > 96%, in a dose-dependent manner and the concentration of a drug that gives half-maximal response (EC50) values of 0.90 (F-5) and 0.73 (FAc-1) μg/ml. Virucidal assay, where 20 μg/ml of F-5 showed a strong effect, reducing chikungunya virus infectivity by up to 88%. Associating F-5 and FAc-1 with ribavirin at suboptimal dosages produced a synergistic effect that completely inhibited viral replication. Time addition experiments demonstrate that F-5 was greater than 85% even if added at 16 h after infection. Our results indicate that these natural products have an excellent inhibitory potential against chikungunya virus replication. |
| Databáze: | OpenAIRE |
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