An orientation-independent DIC microscope allows high resolution imaging of epithelial cell migration and wound healing in a cnidarian model

Autor:	Michael Shribak, Jocelyn E. Malamy
Rok vydání:	2018
Předmět:	0301 basic medicine Histology Microscope Chemistry Epithelial cell migration 01 natural sciences Epithelium Pathology and Forensic Medicine law.invention 010309 optics 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Clytia hemisphaerica Differential interference contrast microscopy law 0103 physical sciences Monolayer medicine Biophysics Wound healing Preclinical imaging
Zdroj:	Journal of Microscopy. 270:290-301
ISSN:	0022-2720
DOI:	10.1111/jmi.12682
Popis:	Epithelial cell dynamics can be difficult to study in intact animals or tissues. Here we use the medusa form of the hydrozoan Clytia hemisphaerica, which is covered with a monolayer of epithelial cells, to test the efficacy of an orientation-independent differential interference contrast microscope for in vivo imaging of wound healing. Orientation-independent differential interference contrast provides an unprecedented resolution phase image of epithelial cells closing a wound in a live, nontransgenic animal model. In particular, the orientation-independent differential interference contrast microscope equipped with a 40x/0.75NA objective lens and using the illumination light with wavelength 546 nm demonstrated a resolution of 460 nm. The repair of individual cells, the adhesion of cells to close a gap, and the concomitant contraction of these cells during closure is clearly visualized.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9af1aeb2b3ac3d53fea59d02eb94aa4c https://doi.org/10.1111/jmi.12682 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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