Preparation of Spheroplasts of Schizosaccharomyces pombe

Autor:	Irvin Isenberg, Douglas P. Dickinson
Rok vydání:	1982
Předmět:	chemistry.chemical_classification biology fungi Spheroplast biology.organism_classification Microbiology Enzyme Biochemistry chemistry Stationary phase Organelle Schizosaccharomyces pombe Cell fractionation
Zdroj:	Microbiology. 128:651-654
ISSN:	1465-2080 1350-0872
DOI:	10.1099/00221287-128-3-651
Popis:	Summary: A method is described for the conversion of Schizosaccharomyces pombe cells to spheroplasts using Novozym 234, a commercially available enzyme preparation. Both exponentially growing and stationary phase cells can be quantitatively converted to spheroplasts on a large scale. A means of disrupting these spheroplasts is described which gives an efficient release of organelles. The resulting homogenate is suitable for subcellular fractionation. The spheroplasts can also be regenerated to give dividing cells, thus providing an approach to genetic manipulation in S. pombe.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9aef9293bd01f66b1298d870cd2adba9 https://doi.org/10.1099/00221287-128-3-651 Zobrazit plný text záznamu