Presence d'acides polyinsatures dans une bacterie: isolement, a partir des lipides de Mycobacterium phlei, d'acide hexatriacontapentaene-4,8,12,16,20oique et d'acides analogues
| Autor: | Jean-Claude Promé, Cécile P. Asselineau, Henri Montrozier |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry biology.organism_classification Mass spectrometry Biochemistry Thin-layer chromatography chemistry.chemical_compound Biosynthesis lipids (amino acids peptides and proteins) Gas chromatography Saponification Bacteria Mycobacterium phlei Polyunsaturated fatty acid |
| Zdroj: | European Journal of Biochemistry. 10:580-584 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1969.tb00728.x |
| Popis: | So far, bacteria were considered as devoid of polyunsaturated fatty acids. In spite of this assumption, the lipids of Mycobacterium phlei have a relatively high content (about 5% of the total free lipids) of acids with the general formula CH3–(CH2)n (CH=CH–CH2–CH2)m–CO2H (I), where n = 14 or 12 and m = 5, 6 or 4. The main component of this mixture is hexatriaconta 4,8,12,16,20-pentanoic acid. These acids can be isolated by saponification of the crude lipids, and detected by gas chromatography and thin layer chromatography on silver nitrate-impregnated silicagel. Their structure was established by means of spectrographic methods (infrared and ultraviolet spectrography, nuclear magnetic resonance and mass spectrometry), by study of the products obtained after oxidative degradation, and by comparison of the hydrocarbons prepared from these acids with synthetic hydrocarbons. The polyunsaturated acids are present in several complex lipids, including only a minor fraction of phospholipids. Because of their structure, the acids I belong to a new type of natural polyunsaturated fatty acids and might require a peculiar mode of biosynthesis, involving a repetition process. |
| Databáze: | OpenAIRE |
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