Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop (Placopecten magellanicus) and the Iceland Scallop (Chlamys islandica)

Autor:	D. I. Cook, K. S. Naidu, D. L. Roddick, Elen Kenchington, Eleftherios Zouros
Rok vydání:	1993
Předmět:	biology Ecology Zoology Aquatic Science biology.organism_classification Bivalvia Placopecten magellanicus Chlamys islandica Genetic marker Scallop Population management Adductor muscles Mollusca Ecology Evolution Behavior and Systematics
Zdroj:	Canadian Journal of Fisheries and Aquatic Sciences. 50:1222-1228
ISSN:	1205-7533 0706-652X
DOI:	10.1139/f93-138
Popis:	Three biochemical techniques were applied to adductor muscles of Placopecten magellanicus and Chlamys islandica, two commercially important scallops, to search for species-specific genetic markers. Allozyme electrophoresis identified two enzyme systems, mannose phosphate isomerase and glutamic oxaloacetate transaminase, which appear to be diagnostic. Sequencing of the 18S rRNA gene in each species showed 15 nucleotide differences over 1815 base pairs. Digestion of the gene with the restriction enzyme XHO I released two fragments in Placopecten and three in Chlamys. All three techniques could be developed for management purposes as part of an enforcement programme to identify illegally caught scallops.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9adb9e95ec06d9a003c3810fc5281a2c https://doi.org/10.1139/f93-138 Zobrazit plný text záznamu