Determination of pyrimidine cyclobutane dimers by electrospray ionization/ion trap mass spectrometry

Autor:	Scott A. McLuckey, Roswitha S. Ramsey, Gary L. Glish, G. J. Van Berkel
Rok vydání:	1992
Předmět:	Electrospray ionization Inorganic chemistry Extractive electrospray ionization Analytical chemistry Pyrimidine dimer Mass spectrometry Biochemistry Sample preparation in mass spectrometry Ion source Cyclobutane chemistry.chemical_compound chemistry Molecular Medicine Spectroscopy Ambient ionization
Zdroj:	Biological Mass Spectrometry. 21:347-352
ISSN:	1096-9888 1052-9306
DOI:	10.1002/bms.1200210705
Popis:	A straightforward approach for determining the cyclobutane pyrimidine dimers, thymine-thymine, uracil-thymine, and uracil-uracil, based upon electrospray ionization/ion trap mass spectrometry is described. These compounds are detected as the sodiated molecules, [M + Na]+, the response for which is maximized by adding low concentrations of sodium salts to the electrospray solutions. Under these conditions subnanogram levels of the dimers are determined by flow injection analysis. Structural information is obtained by collision-induced dissociation of the lithium adducts, [M + Li]+, which are formed by adding lithium salts to the solvents.
Databáze:	OpenAIRE
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