Effect of cytokines on prostaglandin E2 and prostacyclin production in primary cultures of human myometrial cells
| Autor: | Herbert M. Todd, Vijaya L. Dundoo, William R. Gerber, Joseph J. Baldassare, Carrie A. Cwiak, Frank Hertelendy |
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| Rok vydání: | 1996 |
| Předmět: |
Agonist
medicine.medical_specialty medicine.drug_class business.industry Obstetrics and Gynecology Prostaglandin Radioimmunoassay Prostacyclin Cycloheximide Receptor antagonist chemistry.chemical_compound Endocrinology chemistry Internal medicine medicine lipids (amino acids peptides and proteins) Prostaglandin E2 Protein kinase A business medicine.drug |
| Zdroj: | The Journal of Maternal-Fetal Medicine. 5:161-167 |
| ISSN: | 1520-6661 1057-0802 |
| DOI: | 10.1002/(sici)1520-6661(199607/08)5:4<161::aid-mfm1>3.0.co;2-i |
| Popis: | The objective of this study was to characterize interleukin-1, -6, and -8 (IL-1-, IL-6-, and IL-8)-induced prostacyclin (PGI2 as 6-keto PGF1α) and prostaglandin E2 (PGE2) production in primary cultures of human myometrial cells. Prostaglandins (PGs) released into the culture media were quantitated by specific radioimmunoassays. IL-1, but not IL-6 or IL-8, caused a dose- and time-dependent increase in the production of both PGI2 and PGE2. Half-maximally stimulating doses (EC50) of IL-1 were about 0.1 ng/ml, and maximal responses were observed at 1–10 ng/ml, amounting to 15- to 23-fold increases over unstimulated controls. The action of IL-1 was greatly potentiated by the protein kinase C-activating phorbol ester, TPA, and inhibited by actinomycin D and cycloheximide. IL-1-induced PG production was also suppressed by dexamethasone, by the natural IL-1 receptor antagonist (IL-1ra), and by transforming growth factor1β (TGF1β). It is concluded that IL-1 is a potent agonist of PG synthesis in human myometrial c... |
| Databáze: | OpenAIRE |
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