Regulatory effect of humoral milieu on the viral DNA and surface antigen expression of hepatitis B virus (HBV) in vitro
| Autor: | Sang Kyou Lee, Jee Eun Yang, Jin Su Shin, Chun Chang Ho, Bo Young Shin, Jae Seung Moon, Soo Ki Kim, Jee Sang Yoon, Hong Jai Lee |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis Toxicology medicine.disease_cause Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Antigen In vivo Hepatitis B virus HBV medicine General Pharmacology Toxicology and Pharmaceutics Dna viral Hepatitis B virus Liver infection biology Chemistry Public Health Environmental and Occupational Health Molecular biology digestive system diseases In vitro 030104 developmental biology 030220 oncology & carcinogenesis biology.protein sense organs Antibody |
| Zdroj: | Molecular & Cellular Toxicology. 15:123-128 |
| ISSN: | 2092-8467 1738-642X |
| Popis: | While a few virions of hepatitis B virus (HBV) are sufficient to liver infection in vivo, even a large quantity of virion is unable to enter into hepatoma cells in vitro. To solve this paradox, we explored if humoral milieu such as serum or culture media, and its constituents, and pH would regulate the viral DNA and surface antigen expression of HBV in vitro. The viral DNA and surface antigen expression of HBV are significantly more stable in human serum than in mouse serum, and Dulbecco’s Modified eagle Medium (DMEM). In line, the increase of anti-HBcAg antibody in mouse serum, and DMEM implies that the virion was cleared in non-host humoral milieu. Further, lipid analysis revealed higher levels of lipids in human serum than those in mouse serum, and DMEM. Lipid removal analysis showed the decreased level of HBV DNA and surface antigen expression in human and mouse serum. In electrolytes analysis, human serum contained lower level of potassium than mouse serum. Last, on the viral DNA and surface antigen expression of HBV, the alkalinity of DMEM was more effective than neutral pH. Collectively, this study indicates that humoral milieu would regulate the viral DNA and surface antigen expression of HBV in vitro. This is the first report to regulate HBV growth/viability via simple manipulation of host humoral milieu. |
| Databáze: | OpenAIRE |
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