Penicillin-specific antibodies: Monoclonals versus polyclonals in ELISA and in an optical biosensor
| Autor: | Eric Cox, Bruno Goddeeris, Kristien Bonroy, Patricia Cliquet |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Chromatography
biology Chemistry medicine.drug_class Immunology technology industry and agriculture macromolecular substances biochemical phenomena metabolism and nutrition Monoclonal antibody Benzylpenicillin Dicloxacillin Molecular biology Penicillin Cloxacillin Polyclonal antibodies Ampicillin medicine biology.protein Agronomy and Crop Science Biosensor Food Science medicine.drug |
| Zdroj: | Food and Agricultural Immunology. 16:101-115 |
| ISSN: | 1465-3443 0954-0105 |
| DOI: | 10.1080/09540100500139239 |
| Popis: | Two penicillin-specific monoclonal antibodies mAb 19C9 and mAb 9H3 and the penicillin-specific polyclonal antibodies pAb K2 were evaluated for their use in a competitive ELISA and in the BIAcore™ optical biosensor. In the ELISA, an ampicillin-protein conjugate was used as a coating molecule. For the biosensor assay, ampicillin was immobilized on a CM5 chip. With both monoclonal antibodies and in both test systems, ampicillin, amoxicillin and benzylpenicillin were better recognized than oxacillin, cloxacillin and dicloxacillin. Because the reproducibility was better in the biosensor (CV = 1.6%) than in the ELISA (CV = 8.9%), the limit of detection for ampicillin in buffer solution using mAb 19C9 was lower in the biosensor (46 ng ml−1) as compared to the ELISA (356 ng ml−1). Ampicillin can thus be detected below the MRL (50 ng ml−1) in the biosensor assay but not in the ELISA. Both the ELISA and biosensor assay using the polyclonal antibodies pAb K2 were more sensitive as compared to the assays wit... |
| Databáze: | OpenAIRE |
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