Determination of intact molecular species of bovine milk 1,2-diacyl-sn-glycero-3-phosphocholine and 1,2-diacyl-sn-glycero-3-phosphoethanolamine by reversed phase HPLC, a multivariate optimization
| Autor: | N. U. Olsson, P. Kaufmann |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Chromatography Organic Chemistry Clinical Biochemistry technology industry and agriculture Phospholipid Fatty acid Reversed-phase chromatography Glycerophospholipids Biochemistry Analytical Chemistry Chemometrics chemistry.chemical_compound chemistry Glycerophospholipid Glycerol lipids (amino acids peptides and proteins) Phosphocholine |
| Zdroj: | Chromatographia. 35:517-523 |
| ISSN: | 1612-1112 0009-5893 |
| Popis: | The molecular species profile, representing the fatty acid combinations on the glycerol backbone, is the most fundamental description of an intact glycerophospholipid class and provides a key to understanding its functional characteristics. We have developed a powerful RP-HPLC method for the separation of molecular species, including so-called “critical pairs”, of natural mixtures of 1,2-diacyl-sn-glycero-3-phosphocholine 1,2-diacyl-sn-glycero-3-phosphoethanolamine from bovine milk and other natural sources. We have used a novel multivariate development and optimization strategy to locate the region of optimum separation conditions within a prechosen domain of the chromatographic parameter space. |
| Databáze: | OpenAIRE |
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