Identification of full length β-amyloid precursor protein in human neuronal and non-neuronal cell culture supernatant: a possible extracellular source for the generation of Aβ
| Autor: | Kelly J. Conn, Gregoris Papastoitsis, Barbara Meckelein, Carmela R. Abraham |
|---|---|
| Rok vydání: | 1994 |
| Předmět: | |
| Zdroj: | Amyloid. 1:232-239 |
| ISSN: | 1744-2818 1350-6129 |
| DOI: | 10.3109/13506129409146114 |
| Popis: | β protein (Aβ) which is deposited in the amyloid plaques and vasculature in brains of Alzheimer's Disease (AD) patients is a 39 to 43 amino acid peptide proteolytically derived from the amyloid precursor protein (AβPP). Three major isoforms are expressed in the brain: AβPP751 and AβPP770 which contain a Kunitz-like protease inhibitor domain (KPI), and AβPP695 To date it is still unknown which AβPP isoforms are the precursors of Aβ, which proteolytic pathways are involved in its production, and if the processing occurs intracellularly and/or extracellularly. We now report the identification, by Western blot analysis, of an Aβ-containing AβPP protein which co-migrates with full length recombinant AβPP751 in the culture supernatant of two human neuroblastoma cell lines and in one human kidney cell line. This protein is recognized with six different antibodies towards AβPP targeting intracellular, extracel lular, and the Aβ region of AβPP. The immunodetection of this Aβ precursor is shown to be specific by ab... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|