Effects of benzo(a)pyrene, 3,3′,4,4′-tetrachlorobiphenyl and 2,2′,4,4′,5,5′-hexachlorobiphenyl on the xenobiotic-metabolizing enzymes in the mussel (Mytilus galloprovincialis)
Autor: | L.W. Robertson, X.R. Michel, J. F. Narbonne, P. Suteau |
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Rok vydání: | 1993 |
Předmět: |
chemistry.chemical_classification
Oxidase test animal structures biology organic chemicals Health Toxicology and Mutagenesis Cytochrome P450 Glutathione Aquatic Science complex mixtures chemistry.chemical_compound Enzyme Benzo(a)pyrene chemistry Biochemistry embryonic structures Benzopyrene polycyclic compounds biology.protein Pyrene Epoxide hydrolase |
Zdroj: | Aquatic Toxicology. 27:335-344 |
ISSN: | 0166-445X |
DOI: | 10.1016/0166-445x(93)90062-6 |
Popis: | Benzo(a)pyrene (BaP), 3,3′,4,4′-tetrachlorobiphenyl (TCB) and 2,2′,4,4′,5,5′-hexachlorobiphenyl were tested for their ability to induce hepatic xenobiotic metabolizing enzymes in a marine mussel. TCB and BaP (two planar molecules), but not HCB (a bulky PCB), increased phase I enzyme parameters including the P-450 content, NADPH cytochrome c reductase activity and BaP oxidase activity. The glutathione S-transferase activity and glutathione content increased in HCB-treated mussels and decreased or remained the same as in the control in BaP- and TCB-exposed animals. Epoxide hydrolase (EH) activity increased in BaP- and HCB- but not in TCB-treated groups. These results show that, as in fish, only 3-methylcholanthrene P-450-type inducers (BaP and TCB, a non-ortho-substituted PCB) and not the phenobarbital P-450-type inducer (HCB, a di-ortho-substituted PCB) are effective inducers of phase I enzymes in mussel at the doses tested. Phase II activities were more sensitive to BaP and HCB than to TCB treatment. |
Databáze: | OpenAIRE |
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