Alterations in phagocytosis and the binding of immunoglobulin to bovine neutrophils after chemotaxis

Autor:	R. R. Peters, Leanne M. Berning, M. J. Paapel
Rok vydání:	1991
Předmět:	medicine.medical_specialty Hematology Phagocytosis Chemotaxis Biology IgM binding medicine.disease_cause Pathology and Forensic Medicine Andrology In vivo Staphylococcus aureus Internal medicine Immunology medicine biology.protein Anatomy Antibody Immunoglobulin binding
Zdroj:	Comparative Haematology International. 1:129-134
ISSN:	1618-565X 0938-7714
DOI:	10.1007/bf00515659
Popis:	Twelve nulliparous Holstein heifers, approximately 12 months of age, were given intramammary injections of 5 μg Escherichia coli endotoxin in sterile saline. Peripheral blood and mammary neutrophils were collected 14 h post-injection and evaluated for endogenous binding of immunoglobulins G1, G2, A and M. Isolated blood neutrophils from four of the above heifers were placed into a modified Boyden chemotaxis chamber, allowed to migrate for 3 h, and then evaluated for endogenous immunoglobulin binding. Phagocytosis was evaluated flow cytometrically for neutrophils from four heifers of the same group. Comparisons were made between neutrophils isolated from peripheral blood and neutrophils which had undergone chemotaxis in vitro. Percentage of neutrophils fluorescing and relative fluorescence intensity of endogenous binding of IgG1, and IgG2 were marginally increased (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9a9f61ea5038401a7ce1a50c5d7832d2 https://doi.org/10.1007/bf00515659 Zobrazit plný text záznamu Full text from SpringerLink