Site-specific incorporation of 5-methylaminomethyl-2-thiouridine and 2-thiouridine(s) into RNA sequences

Autor:	Piotr Leonczak, Jakub Pięta, Agnieszka Tomaszewska, Grazyna Leszczynska, Andrzej Malkiewicz
Rok vydání:	2012
Předmět:	Chemistry Stereochemistry Organic Chemistry RNA Sequence (biology) medicine.disease_cause Biochemistry Thiouridine Solid-phase synthesis Drug Discovery Transfer RNA medicine Oligoribonucleotides 5-methylaminomethyl-2-thiouridine Escherichia coli
Zdroj:	Tetrahedron Letters. 53:1214-1217
ISSN:	0040-4039
Popis:	We present a reproducible protocol for the site-specific incorporation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) into a model RNA fragment and, together with 2-methyladenosine (m2A), into the native sequence of the Escherichia coli tRNAGlu2 anticodon arm (E. coli ASLGlu2). This approach is also utilized for the synthesis of oligomers modified with multiple 2-thiouridines.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9a9c6036ae416232587679a5b34a86d9 https://doi.org/10.1016/j.tetlet.2011.12.079 Zobrazit plný text záznamu Full Text from ScienceDirect