Magnetic mesoporous enzyme-silica composites with high activity and enhanced stability
| Autor: | Yuxiao Feng, Shu Yue, Linbo Li, Jiandong Cui, Tao Lin, Yamin Zhao, Ronglin Liu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
General Chemical Engineering
Nanoparticle 02 engineering and technology Phenylalanine ammonia-lyase 01 natural sciences Catalysis Inorganic Chemistry Organic chemistry Fourier transform infrared spectroscopy Waste Management and Disposal chemistry.chemical_classification 010405 organic chemistry Renewable Energy Sustainability and the Environment Organic Chemistry Polymer 021001 nanoscience & nanotechnology Pollution humanities 0104 chemical sciences Fuel Technology chemistry Chemical engineering Biocatalysis Magnetic nanoparticles 0210 nano-technology Mesoporous material Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 91:1905-1913 |
| ISSN: | 0268-2575 |
| Popis: | BACKGROUND Encapsulation of enzymes in biomimetic silica seems to be a promising immobilization method due to mild processing conditions and short preparation time. However, biomimetic silica particles may be considered too small for industrial applications because they result in difficult recovery. Furthermore, the use of R5 peptide or polymers as catalyst usually causes protein denaturation. To overcome these drawbacks, a new stable and recyclable biocatalyst based on co-encapsulation of magnetic nanoparticles and phenylalanine ammonia lyase (PAL) in biomimetic silica has been prepared using enzyme-assisted direct condensation reactions of silicon oxide with tetramethoxysilane (TMOS). RESULTS Under optimal conditions (10 mg mL−1 nanoparticles, 4.5 U mL−1 PAL, 0.8 mol L−1 TMOS), the highest activity recovery (52%) of encapsulated PAL was obtained. Compared with conventional PAL encapsulated in biomimetic silica (28%), the activity recovery was increased 1.86-fold. TEM, CLSM, magnetisation measurements, and FTIR confirmed that the magnetic nanopaticles and PAL had been co-encapsulated into the biomimetic silica. The resulting immobilized PAL exhibited higher activity and stability than native PAL, and the immobilized PAL can be easily separated by applying a magnetic field. CONCLUSIONS This work provides a convenient strategy to prepare stable and easy recycled biocatalyst with excellent catalytic performance. © 2015 Society of Chemical Industry |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text