| Popis: |
ToxAa necrotrophic effector protein, is known to be present in the genome of fungal species likeParastagnospora nodorum,Pyrenophora tritici-repentisandBipolaris sorokiniana. Tsn1is the sensitivity gene in the host whose presence indicates more susceptibility toToxAcarrying pathogen, andToxA-Tsn1delivers an inverse gene-for-gene relationship. The present study involved cloning and expressing theToxA1gene fromB. sorokiniana. It was found that the amplicon exhibited an expected product size of 471 bp. Sequence analysis of theToxA1nucleotide sequence revealed the highest identity, 99.79%, withPy. tritici-repentis. The protein expression analysis showed peak expression at 16.5kDa. Phylogenetic analysis ofToxA1sequence from all theBipolarisisolates formed independent clade along withPy. tritici-repentisand diverged fromP. nodorum.ToxA-Tsn1interaction was studied in 18 wheat genotypes (11Tsn1and 7tsn1) at both the seedling and adult stage, validating the inverse gene-for-gene relationship, as the toxin activity was highest in the K68 genotype (Tsn1) and lowest in WAMI280 (tsn1). This shows a path towards more genomic regions involved in resistance to spot blotch disease, which could be further explored. |
