Evalution of Antioxidant Properties of Salivary Proteins Using Electron Spin Resonance Technique
| Autor: | Gotaro Shiota, Masaichi-Chang-il Lee, Tomoko Komatsu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Antioxidant Chemistry Superoxide medicine.medical_treatment No scavenging Biochemistry law.invention chemistry.chemical_compound Nuclear magnetic resonance law Physiology (medical) medicine Salivary Proteins Hydroxyl radical Electron paramagnetic resonance Scavenging |
| Zdroj: | Free Radical Biology and Medicine. 100:S102 |
| ISSN: | 0891-5849 |
| Popis: | Salivary proteins are armed with various defense mechanisms. We investigated the effect of salivary proteins on the scavenging effects of reactive oxygen species (ROS) by electron spin resonance (ESR) techniques. Histatin1, 3, 5, PRP1, 2, 3, 4, 30 residues of N-terminal of PRP1 (30r-PRP1), and statherin were subjected to this study. These human salivary proteins were kindly provided by Prof. F. G. Oppenheim (Boston University, MA, USA). Hydroxyl radical (●OH) and superoxide (O2●–) was evaluated using ESR to evaluate the antioxidant properties of salivary proteins. All of histatins and PRPs were markedly inhibited HO· generation. There is no scavenging effect on O2●–generation in all of histatins and PRPs. There is no scavenging effect on ROS generation in statherin. It was revealed that both histatins and PRPs had anti-oxidant properties against ●OH, while statherin had no anti-oxidant properties. |
| Databáze: | OpenAIRE |
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