Sequence-specific endonucleases in strains of Anabaena and Nostoc

Autor:	C. Peter Wolk, J. Korsuize, Avigad Vonshak, A. de Waard, M. G. C. Duyvesteyn
Rok vydání:	1983
Předmět:	chemistry.chemical_classification Cyanobacteria Nostoc Lysis biology Anabaena General Medicine biology.organism_classification Biochemistry Microbiology Molecular biology Endonuclease Restriction enzyme Enzyme chemistry Isoschizomer Genetics biology.protein Molecular Biology
Zdroj:	Archives of Microbiology. 134:276-281
ISSN:	1432-072X 0302-8933
DOI:	10.1007/bf00407802
Popis:	The complements of restriction endonucleases of 12 strains of cyanobacteria were determined in cell-free extracts, and were compared with the complements of restriction activities assessed by measuring the relative efficiencies of plating of cyanophages on those cyanobacteria. The hosts which were susceptible to all of the phages contained endo R · AvaI and endo R · AvaII, and in several cases probably endo R · AvaIII, or isoschizomers of these enzymes. Three hosts which were lysed by only a subset (1 or 3) of the phages contained different restriction endonuclease. Anabaena sp. PCC 7120 showed apparent phenotypic restriction of phage An-22 grown in hosts with (isoschizomers of) AvaI, II and III, but no corresponding endonuclease has yet been detected in vitro. Nostoc sp. ATCC 29131 (PCC 6705) was found to contain a restriction enzyme, NspBII, with hitherot unknown specificity, C(A/C)GC(T/G)G.
Databáze:	OpenAIRE
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