An Integrated Whole-Cell Detection Platform for Heavy Metal Ions
| Autor: | Pablo Morales Navarrete, Chiye Zhang, Jie Yuan, Saika Siddiqui |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Detection limit
Cadmium Materials science Metal ions in aqueous solution 010401 analytical chemistry Detector chemistry.chemical_element Nanotechnology Genetically modified bacteria Contamination 01 natural sciences 0104 chemical sciences Electrochemical gas sensor chemistry Electrical and Electronic Engineering Whole cell Instrumentation |
| Zdroj: | IEEE Sensors Journal. 20:4959-4967 |
| ISSN: | 2379-9153 1530-437X |
| DOI: | 10.1109/jsen.2020.2964642 |
| Popis: | Heavy metal ions, such as Pb2+ and Cd2+, are main sources of water quality contaminants. Whole-cell detection has recently been actively researched to use genetically modified bacteria to sense the presence of heavy metal ions in water or soil. Compared to common cell-free methods, such as immunosensor and electrochemical sensor, whole-cell sensors need simple sample preparation and can continuously sense metal contaminants in the environment with the cell culture. Nonetheless, whole-cell sensors so far have demonstrated sensitivity too low for water quality monitoring. In this work, we developed an integrated platform based on whole-cell detection. Our genetically-modified E. coli bacteria has a good specificity on lead and cadmium. The platform integrates the light source, cultured cells and the detector for the first time. The integrated platform shows good sensitivity. Limits of detection for Pb2+ and Cd2+ are 518 ppb and 44.8 ppb respectively. |
| Databáze: | OpenAIRE |
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