Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer Cells
| Autor: | Rahmi Annisa, Ferry Sandra, Roihatul Mutiah, Ana Yuliah Rahmawati, Farenza Okta Kirana |
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| Rok vydání: | 2020 |
| Předmět: |
Arcangelisia flava
Antioxidant Traditional medicine Chemistry Colorectal cancer Alkaloid medicine.medical_treatment medicine.disease 01 natural sciences Thin-layer chromatography 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences 0302 clinical medicine Active agent 030220 oncology & carcinogenesis medicine Cytotoxicity IC50 |
| Zdroj: | Indonesian Journal of Cancer Chemoprevention. 11:84 |
| ISSN: | 2355-8989 2088-0197 |
| DOI: | 10.14499/indonesianjcanchemoprev11iss2pp84-89 |
| Popis: | Yellow root (Arcangelisia flava (L.) Merr.) has been scientifically known to have potential as an antimalarial, antibacterial, antioxidant, and anticancer. The purpose of this study was to determine the profile of alkaloid content and cytotoxicity of yellow root extract from several regions in Kalimantan. The alkaloid content was tested using the thin layer chromatography (TLC) method with dragendorf reagent. Cytotoxic in vitro test was conducted against WiDr colorectal cancer cells using the 3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide (MTT) assay. Yellow roots were collected from Samarinda city, Banjarmasin city, Barito Timur regency, Malinau district, and Balikpapan City. The MTT inhibitory concentration 50 (IC50) of yellow root extracts were 573.308 μg/mL; 582.857 μg/mL; 296.326 μg/mL; 114.119 μg/mL; and 320.162 μg/mL respectively. Results of the compound identification indicated that alkaloid was found in A. flava from all regions. Alkaloids of A. flava extract should be investigated further in order to find possible active agent that could decrease the viability of WiDr colorectal cancer cells.Keywords: Arcangelisia flava, Borneo, colorectal cancer, Kalimantan, WiDr cells. |
| Databáze: | OpenAIRE |
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