| Popis: |
An isolate of Streptomyces decoyicus was identified by the sequencing of 16S rRNA gene. It was grown on solid media (250 ml) and secondary metabolites were extracted with n-butanol (100 ml). The extract was dried and run in a sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE, 10%). Two main bands obtained were sliced and the metabolites were regained in n-butanol (100µl). These two samples were then identified by gas-chromatography-mass spectrometry (GC-MS), and Fourier-transform infrared spectroscopy (FT-IR). The results demonstrated that tromethamine- and 1-dodecanol were the main constituents (band 1: 61% and 17.7%; band 2: 41% and 54%, respectively). This finding maintained that the isolate of Streptomyces decoyicus produced high amounts tromethamine- and 1-dodecanol-like compounds under the conditions investigated. |
