Conformational energetics of insulin in interpolyelectrolyte complexes insulin-poly(methylaminophosphazene) under near-physiological conditions
| Autor: | Tatiana V. Burova, Valerij Ya. Grinberg, Alexander S. Dubovik, Natalia V. Grinberg, E. D. Shibanova |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Polymers and Plastics Insulin medicine.medical_treatment Organic Chemistry Ionic bonding 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Polyelectrolyte 0104 chemical sciences law.invention Crystallography Differential scanning calorimetry law Ionic strength Materials Chemistry Recombinant DNA medicine Turbidimetry 0210 nano-technology Stoichiometry |
| Zdroj: | Polymer. 85:28-36 |
| ISSN: | 0032-3861 |
| Popis: | Interaction of a synthetic biodegradable polymer, poly(methylaminophosphazene), (PMAP) with human recombinant zinc insulin at pH 7.4 and different ionic strengths was studied by means of turbidimetry, phase analysis and high-sensitivity differential scanning calorimetry. The formation of stoichiometric (insoluble) and non-stoichiometric (soluble) interpolyelectrolyte Insulin-PMAP complexes was detected. It was shown that binding of zinc insulin to PMAP is accompanied by conversion of the protein to its zinc-free form as a result of the Zn+2 release from the insulin native structure. The conformational stability of the bound zinc-free insulin is defined by composition of the complexes. It increases in case of the non-stoichiometric complexes and decreases in the stoichiometric ones. We found that soluble Insulin-PMAP complexes revealed thermoresponsive properties within physiological range of temperature and ionic strength. |
| Databáze: | OpenAIRE |
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