Isolation and physicochemical characterization of highly polymeric cotton-plant nuclear DNA
| Autor: | G. F. Kasymova, M. Karimov, V. K. Burichenko |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
chemistry.chemical_classification
Circular dichroism Molecular mass Plant Science General Chemistry General Biochemistry Genetics and Molecular Biology Nuclear DNA Transformation (genetics) chemistry.chemical_compound chemistry Ionic strength Biophysics Organic chemistry Nucleotide Ultracentrifuge DNA |
| Zdroj: | Chemistry of Natural Compounds. 20:606-609 |
| ISSN: | 1573-8388 0009-3130 |
| DOI: | 10.1007/bf00580076 |
| Popis: | A highly polymeric fraction of nuclear DNA has been isolated from two-day shoots of the cotton plant. The DNA has been characterized by the methods of circular dichroism (CD), thermal denaturation, and ultracentrifugation. It has been established that the DNA preparation isolated has a molecular mass of 18·106 daltons, melts at 85.5°C in a fairly narrow interval, and reveals no heterogeneity of its intramolecular nucleotide composition. It has been shown by the CD method that in a solution of the cotton-plant DNA with relatively low ionic strength there is a tendency to the transformation of the B form into the C form. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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