Development of an in vitro dermis equivalent model using collagen-based scaffold

Autor: Janice Rodrigues Perussi, Thayz F.L. Morais, Ana Maria de Guzzi Plepis, Anna Cecília B. Oliveira, Priscila Fernanda Campos de Menezes
Rok vydání: 2015
Předmět:
Zdroj: Photodiagnosis and Photodynamic Therapy. 12:350
ISSN: 1572-1000
Popis: Photodynamic therapy (PDT) is a technique for the treatment of cancer and other diseases based on the administration of a photosensitizer (PS) which is activated by irradiation of the tumor area with visible light in the presence of oxygen producing highly reactive oxygen species (ROS). Chlorins presents high absorption around 650–670nm, strong ability to generate ROS and low cytotoxicity in the absence of light. In this study the synthesis of two chlorin derivatives sterically prevented from self-aggregation was carried out. The compounds were prepared by the Diels-Alder reaction between protoporphyrin IX dimethyl ester and 1-(2-hydroxyethyl)-1H-pyrrole-2,5-dione. Singlet oxygen quantum yield of the chlorin CHL-OH-A was =0.49±0.04 and =0.43±0.02 for the CHL-OH-B when compared with methylene blue ( =0.52). Photobleaching studies showed low photodegradation (∼10%) after irradiation at the Soret band for 10min at 50mW. The Log P of the CHL-OH-A was 1.31 and 1.55 for the CHL-OH-B therefore they are lipophilic. The cytotoxic studies of the chlorins in tumoralHep-2 cells after irradiation at 660nm, showed IC50of24.6 (A) and19.3nmolL-1 (B) atdoses3 J cm−2 while at6 J cm-2 were8.51and6.61nmol L−1 respectively, suggesting that thesenewcompounds are potential candidates to photosensitizers.
Databáze: OpenAIRE
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