| Popis: |
Publisher Summary This chapter provides an overview of phosphatidylcholine metabolism in signal transduction. Although the hepatocyte is not an inflammatory cell, there is reason to believe that many of the signaling pathways found in the hepatocyte are also present in the other types of cells. The chapter reviews a work on phosphatidylcholine breakdown and hormone action in the rat liver. These studies reflect the tremendous interest in the mechanisms of signal transduction in many laboratories today. The rat liver has many advantages as a system for elucidating the mechanisms of signal transduction. Approximately, 10 g of liver is available from a 250-gm rat; the collagenase perfusion method yields 3–4 gm of hepatocytes from each liver. These hepatocytes are 85–95% viable and respond to a wide variety of agonists, including epinephrine, norepinephrine, vasopressin, angiotensin II, glucagon, epidermal growth factor, insulin, and ATP. |
