Localization of Selenoproteins in the Perinuclear Structure of the Rat Kidney

Autor:	G. Weseloh, A. Graebert, A. Kyriakopoulos, D. Behne, Barbara Hoppe, M. Kühbacher
Rok vydání:	2004
Předmět:	Differential centrifugation Kidney General Chemical Engineering chemistry.chemical_element Golgi apparatus symbols.namesake Electrophoresis medicine.anatomical_structure Biochemistry chemistry symbols Microsome medicine Cell fractionation Instrumentation Reticulum Selenium General Environmental Science
Zdroj:	Instrumentation Science & Technology. 32:221-232
ISSN:	1525-6030 1073-9149
DOI:	10.1081/ci-120028774
Popis:	Investigations were performed on rats to obtain information on the distribution of selenoproteins in the microsomal fraction, especially in the perinuclear structure [endoplasmatic reticulum (ER) and Golgi apparatus] of the kidney. For the determination of the selenium levels instrumental neutron activation analysis (INAA) via 75Se was used. The membrane‐bound selenium‐containing proteins were detected by labeling of rats with 75Se‐selenite, subcellular fractionation of the tissue homogenates, separation of the proteins by SDS‐PAGE, or two‐dimensional electrophoresis and autoradiography of 75Se. In this way, at least 17 membrane‐bound selenium‐containing proteins could be distinguished in the microsomal fraction of the kidney. Of those, six selenium‐containing proteins with molecular masses of about 15, 16, 20, 23–25, 42–44, and 58–60 kDa were found in the ER fraction, which was isolated from the microsomes by differential centrifugation and further purification steps with detergents. The 75Se‐la...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9a122459c41c1d8837341149cbf547db https://doi.org/10.1081/ci-120028774 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.