Molecular detection on the blaKPC gene in the extensively prevalent nosocomial isolates of drug resistant Acinetobacter spp at hospital in southeastern region of Brazil
| Autor: | Mauro A.S. Xavier, M. De Luca, Adriana Amaral Carvalho, Léia Cardoso, Alessandra Rejane Ericsson de Oliveira Xavier |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.drug_class
Antibiotics Drug resistance biochemical phenomena metabolism and nutrition Biology Acinetobacter Amplicon biology.organism_classification Microbiology law.invention Acinetobacter baumannii Multiple drug resistance Antibiotic resistance law medicine General Earth and Planetary Sciences Polymerase chain reaction General Environmental Science |
| Zdroj: | Brazilian Journal of Health and Pharmacy. 1:21-25 |
| ISSN: | 2596-321X |
| Popis: | Acinetobacter baumannii is a multiple drug resistance opportunistic pathogen who has been responsible for severe outbreaks in nosocomial environment. A. baumannii possesses several mechanisms of antibiotic resistance that make it resistant to β-lactamics, mainly due to OXA-type carbapenemases expression, and others antibiotics. The blaKPC gene is an antibiotic resistance gene typical of K. pneumoniae and P. Aeruginosa. In 2009, blaKPC was found in A. baumannii isolates in Puerto Rico and later in Sao Luis, Brazil. The aim of this study is to screening the presence of blaKPC in nosocomial A. baumannii isolates from hospital environment monitoring. The study was approved by the Research Ethics Committee of the Universidade Estadual de Montes Claros under number 852.002/2014. Thirty-one DNA samples of Acinetobacter spp extensively drug resistant isolated from hospitalized patients in the southeast of Minas Gerais state – Brazil and kept at -20°C were used for PCR amplification of blaKPC gene detection. The expected amplicon size of 876 bp was visualized on 1.5% agarose gel stained with ethidium bromide and photographed. Twenty-eight DNA samples of Acinetobacter spp. tested negative to blaKPC gene and three isolates showed PCR expected amplicon of 876 bp. The presence of K. pneumoniae and Acinetobacter spp. in the same hospital environment could results in gene transfer between them. Also, the widespread and increasing uses of carbapenems could accelerate the spread of carbapenems-resistant strains by transferring resistance genes among the enterobacteriaceae and related microorganisms, impacting the public health system. |
| Databáze: | OpenAIRE |
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